PATERNAL MODERATE ALCOHOL CONSUMPTION MODIFIES MOUSE PERI-IMPLANTATION EMBRYO DEVELOPMENT IMPAIRING INNER CELL MASS AND TROPHOBLAST GROWTH AND MORPHOLOGY

FUENTES F; CALVO JC; GOTFRYD LUCILA; SALAMONE G; FONTANA V; SÁNCHEZ MC; CEBRAL E

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC

Previously, we showedthat paternal alcohol intake partially inhibits sperm capacitation, increasesnuclear decondensation rate and deregulates the dynamics of pronucleusformation and fertilization. Aim: To evaluate the effect of paternalalcohol consumption and its consequences on mouse early embryo developmentfocusing on trophoblast (TB) differentiation and inner cell mass (ICM) ascritical embryo stages invading maternal decidua. Methods: CF-1 fertilemales were exposed (treated group, T) or not (control group, C) to 15% (v/v)ethanol in drinking water ad libitum for15 days. They were mated with a fertile nontreated CF-1 female (1:1). Positivemating females were sacrificed at day 2 of gestation to obtain 2 cell embryoswhich, then, were cultured for 7 days. Embryo differentiation, growth and morphologywere evaluated during pre- and peri-implantation phases in vitro. Then, embryos were classified as type A (ICM: protrudingaggregates of compact cells; TB: symmetric monolayer of flat and elongatedcells) or type B (ICM: disaggregated, few scattered or no cells, TB: asymmetrictrophoblast outgrowth). Frequency differences between C and T were tested by Fisher´sexact test. Results: Male alcohol consumption for 15 days delayed theembryo differentiation and growth by detention/fragmentation and altered theembryo morphology. Differences between type A and B distribution in C and Twere statistically significant for ICM and TB in both groups. For ICM: Type A53% C vs 10% T (p