IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Impaired embryo cardiac-placental axis at organogenesis associated with dysregulation of VEGF and its receptors after maternal alcohol consumption.
Autor/es:
ARGANDOÑA F; PALOMINO WA; CEBRAL E; BARBEITO C; VENTUREIRA M; SOBARZO CMA
Lugar:
Puerto Varas
Reunión:
Congreso; Joint Meeting 2017 Latin American Society for Maternal Fetal Interaction and Placenta (SLIMP) Sociedad Chilena de Ciencias Fisiológicas (SCHCF)Puerto Varas. Chile; 2017
Institución organizadora:
Latin American Society for Maternal Fetal Interaction and Placenta (SLIMP) Sociedad Chilena de Ciencias Fisiológicas (SCHCF)
Resumen:
Maternal alcohol consumption leads to congenital diseases and earlypregnancy loss. Previously, we saw delayed embryo growth, increaseddysmorphogenesis, abnormal decidua vascularization and alteredexpression of vascular endothelial growth factor (VEGF) and its receptorsafter perigestational 10% alcohol ingestion at organogenesis.Objectives: Given that VEGF/receptors regulates the embryo cardiacplacentalaxis development, we analysed the embryo cardiac andtrophoblast-placental development and this angiogenic system aftermaternal alcohol consumption.Methods: Ethanol 10% in drinking water was administered to murine CF-1females for 15 days before and up to day 10 of gestation (treated females(TF)). Control females (CF) were administered with drinking water withoutethanol.Western blot, immunohistochemistry (IHC), lasermicrodissectionand qRT-PCR were used.Results: The trophoblastic zone of TF presented increased apoptosis(TUNEL), unaltered proliferation and reduced VEGF expression (CF ¼ 0.23± 0.02 vs TF ¼ 0.11 ± 0.01, Arbitrary units (AU), n ¼ 4, P