IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Rol of the gonadotropin inhibitory hormone(GnIH) in the development of the cichlid fish Cichlasoma dimerus related to feeding and gonadogenesis
Autor/es:
KAZUYOSHI TSUTSUI; PAULA G. VISSIO; FRANCISCO JAVIER TOLEDO SOLIS; DANIELA I. PÉREZ SIRKIN; PAULA G. VISSIO; JULIETA EMILSE SALLEMI; DANIELA I. PÉREZ SIRKIN; TOMÁS H. DELGADIN; JULIETA EMILSE SALLEMI; MARÍA PAULA DI YORIO; TOMÁS H. DELGADIN; MARÍA PAULA DI YORIO; KAZUYOSHI TSUTSUI; FRANCISCO JAVIER TOLEDO SOLIS
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Resumen:
GnIH is a dodecapeptide that belongs to the RFamide family. Itsacts on the pituitary to inhibit gonadotropin (GTH) release in birdsand mammals, but this function in other vertebrates is unclear. Previously,in our lab, we showed that GnIH inhibited GTH and stimulatedgrowth hormone (GH) release in adult of C. dimerus. Wealso studied the ontogeny and we found that GnIH is expressed inneurons of the nucleus olfacto retinalis (NOR) and the nucleus posteriorisperiventricularis (NPP). GnIH immureactive (-ir) fibers werewidely distributed in the brain and other cephalic regions, even inthe pituitary. Our first goal was to study if the expression of GnIHpresents variations related to changes in the feeding and gonadaldevelopment during ontogeny. Our second goal was to evaluatea possible co-expression of GnIH and GnRH variants, and also ifthere were contacts between GnIH fibers and GTH and GH cells.Methodology: larvae were obtained from 4 independent spawnings.Samples were taken from hatching to 85 days post-hatching (dph)and processed for immunohistochemistry/double label immunofluorescenceor real time PCR. Neurons of both nuclei were quantified(repeated measures AN0VA) and measured. We observed thatthe number of neurons in the NOR increased by 17 and 85 dph(p