IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Impaired embryo cardiac-placental axis at organogenesis associated with dysregulation of VEGF and its receptors after maternal alcohol consumption
Autor/es:
ARGANDOÑA F; BARBEITO C; PALOMINO WA; CEBRAL, E; VENTUREIRA, MR; SOBARZO C
Lugar:
Puerto Varas
Reunión:
Simposio; SLIMP-Maternal-Fetal Interaction; 2017
Institución organizadora:
SLIMP
Resumen:
Maternal alcohol consumption leads to congenital diseases and early pregnancy loss.Previously, we saw delayed embryo growth, increased dysmorphogenesis, abnormaldecidua vascularization and altered expression of vascular endothelial growth factor(VEGF) and its receptors after perigestational 10% alcohol ingestion at organogenesis. Objectives: Given that VEGF/receptors regulates the embryo cardiacplacental axis development, we analysed the embryo cardiac and trophoblastplacental development and this angiogenic system after maternal alcohol consumption. Methods: Ethanol 10% in drinking water was administered to murine CF-1 females for 15 days before and up to day 10 of gestation (treated females (TF)). Control females (CF) were administered with drinking water without ethanol. Western blot, immunohistochemistry (IHC), laser microdissection and qRT-PCR were used. Results: The trophoblastic zone of TF presented increased apoptosis (TUNEL), unaltered proliferation and reduced VEGF expression (CF = 0.23 ± 0.02 vs TF = 0.11 ± 0.01, Arbitrary units (AU), n = 4, P