Abnormal endometrial expression of complement regulatory protein decay accelerating factor in women with recurrent implantation failure in cycles of assisted reproduction

PALOMINO WA; VENTUREIRA M; ARGANDOÑA F; DEVOTO L; AGUIRRE C; KOHEN P

Puerto Varas

Congreso; VII Latin American Symposium on Maternal-Fetal Interaction and Placenta - Sociedad Chilena de Ciencias Fisiológicas; 2017

Sociedad Latinoamericana de Interacción Materno-Fetal y Placenta

The control of complement activation within embryo-maternal environment is absolutely required for embryo survival. Complement system components are expressed in endometrium during embryo receptivity phase. The mechanism involved in the recurrent implantation failure (RIF) in assisted reproduction remains unknown. Objective: To assess the expression of complement C3 and complement regulatory proteins: decay accelerating factor (DAF), CD46 and CD59 in the endometria of women with RIF. Methods: RIF was defined as the failure of embryo implantation after three in vitro fertilization (IVF) cycles in which one or two morphological highgrade embryos were transferred. Endometrial biopsies from 30 patients with RIF were obtained six days after progesterone administration during mock hormonal endometrial preparation cycle for embryo transfer. Endometrial samples from 16 fertile women obtained six days after ovulation were included as controls. Proteins were localized by immunohistochemistry and their relative expression determined using histological score (Hscore). The mRNA expression was determined by qRTPCR. To compare the findings non-parametric Mann-Whitney statistical test was applied. Results: Delayed glandular dating with advanced stromal transformation was observed in 40% of RIF. According to Hscore and mRNA levels, DAF expression was significantly low in RIF?s samples (P