IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Bioaccumulation of endosulfan on a cichlid fish: differences between exposure to the active ingredient or a commercial formulation
Autor/es:
DA CUÑA, RODRIGO; SHIMABUKURO, V; MIGLIORANZA, KARINA S.B.; RODRIGUEZ, ENRIQUE; LO NOSTRO, FABIANA
Lugar:
CABA
Reunión:
Congreso; SETAC Latin America 11th Biennial Meeting; 2015
Institución organizadora:
SETAC
Resumen:
The organochlorine pesticide endosulfan (ES) has been restricted or banned worldwide after its inclusion in the list of Persistent Organic Pollutants by the Stockholm Convention. In Argentina, it has been intensively used as a wide range insecticide in crops of high commercial value like soy. Consequently, this pesticide is currently one of the most frequently detected contaminants in the aquatic environment. The pesticide mixture contains two isomers, - and -ES, which can be converted to ES-sulfate (ES-S) by biotic or abiotic oxidation. All 3 compounds exhibit high acute toxicity to aquatic organisms, acting as reproductive disruptors in fish. Toxicological studies focus almost exclusively on the active ingredients (AIs) of pesticides. However, AIs and commercial formulations (CFs) can differ in toxicity, due to additive or synergistic effects of non-specified ?inert? ingredients -adjuvants and additives used to increase solubility, dispersal and/or uptake. The aim of this study was to evaluate the difference in bioaccumulation of waterborne ES after acute exposure to the AI or a CF, in adult male C. dimerus. Fish were exposed to 0.7 g L-1 ES AI (94.99% purity) or a CF (Zebra Ciagro® 35% ES) for 2 weeks under semi-static conditions. After exposure, liver, gonad, gill, brain and muscle samples were collected. Endosulfan -, - and ES-S were quantified by gas chromatography-electron capture detector. Differences in bioaccumulated ES between organs (ng g−1 wet wt) were tested using a Friedman ANOVA analysis for multiple dependent samples. Spearman correlation coefficient was performed between lipid % and bioaccumulation levels. On fish exposed to ES, high levels of ES-S were detected in most organs, save for muscle. Levels of this metabolite were highest in liver and brain of fish exposed to the CF. Regarding ES significant levels were detected in gills and brain of fish exposed to the AI, whereas in males exposed to the CF levels were minimal. Lipid content was highest for liver, followed by testis, brain, gills and muscle. There was a positive correlation between lipid % and ES-S levels in fish exposed to the AI and CF. Obtained results suggest a rapid metabolization of ES isomers to ES-S in fish tissues. Isomers were present in highest amounts in gills, the organ in direct contact to waterborne ES, suggesting recent uptake. The presence of coadyuvants appears to increase bioaccumulation of ES-S, particularly in liver and brain.