In vitro and in vivo effects of Roundup on the ovarian growth of the estuarine crab Neohelice granulata

CANOSA, IVANA; SILVEYRA, GABRIELA; MAC LOUGHLIN, CAMILA; MEDESANI, DANIEL; RODRÍGUEZ, ENRIQUE M.

Ciudad Autónoma de Buenos Aires

Congreso; SETAC LATINAMERICA 11th BIENNAL MEETING; 2015

Sociedad de Toxicología y Química Legal-SETAC Argentina

The effect of Roundup Ultramax® (R-UP), one of the main glyphosate formulation currently used, was evaluated in vitro on ovarian pieces of the estuarine crab Neohelice granulata, during the pre-reproductive period. Small pieces were incubated for 24 h in Medium199, inside a culture chamber held at 27ºC and 5% CO2. Experimental groups comprised a control (vehicle) and three concentrations of R-UP: R-UP1 (0.0025 mg/L), R-UP2 (0.0125 mg/L) and R-UP3 (0.2 mg/L), these concentrations referring to glyphosate as active principle in the commercial formulation. Twelve females were used, each one providing biological material to every treatment (repeated measure design). Tritiated leucine was added to each 2 mL-well, in order to estimate the incorporation of this amino-acid to de novo synthesis of ovarian proteins (i.e., mainly vitellins). At the end of the assay, ovarian pieces were homogeneized, centrifugated and filtered, to finally counting the CPM in the acid precipitable fraction. A significant (p