IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
USE OF THE FLUOROCHROME CALCEIN AS AN IN SITU GROWTH MARKER IN BRACHIDONTES RODRIGUEZII (D?ORBIGNY, 1846)
Autor/es:
TORROGLOSA, MARIA EUGENIA; OJEDA, MARIEL; GIMENEZ, JULIANA
Lugar:
Ciudad de México
Reunión:
Congreso; Mollusca 2014 El Encuentro de las Américas; 2014
Resumen:
A capture- mark and recapture study was done in order to evaluate size increment in the nature. B. rodriguezii inhabits the intertidal zone with differentiated exposition into the water. The flourochrome calcein was used in situ experiment. Field experiment was carried out in Villa Gesell (56° 53´W -37º 16´S) a sandy beach with artificial substrates (fishing dock) during summer. Specimens were collected by hand and calcein solution was applied by immersion during  four hours. Then small cages with the marked mussels inside were attached again to the artificial substrate. After one month, cages were removed. For growth estimations, marked valves were sectioned sagitally, polish and observed through a fluorescence microscope at 450 to 490 nm. Growth rates estimations indicated a differential growth along the substrate, related with the water exposition,. Mussels exposed to the  water only in high tide showed a less increment in length than mussels exposed to the water during the low and high tide. Relationship between growth rates and shell length was best described by a logarithmic function.