IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effects of fluoxetine on the reproductive axis and aggression of the cichlid fish Cichlasoma dimerus (Teleostei, Perciformes).
Autor/es:
LO NOSTRO, F.; DA CUÑA, R.; REY VAZQUEZ, G.; MEIJIDE, F.; HONJI, R.; DORELLE L.; MOREIRA, R.; GENOVESE, G.
Lugar:
Glasgow
Reunión:
Congreso; 23rd Annual Meeting of the Society of Environmental Toxicology and Chemistry (SETAC Europe); 2013
Institución organizadora:
SETAC
Resumen:
Pharmaceuticals are increasingly detected in a variety of aquatic systems. Fluoxetine (FLX) -the active ingredient of the antidepressant Prozac®- is a selective serotonin reuptake inhibitor which can bioaccumulate in wild-caught fish. This has raised concerns over potential disruptive effects of FLX because of the known role of serotonin in the modulation of neuroendocrine function in teleost fish. Some fish exhibit a wide range of behavioral responses associated with their reproduction. Cichlasoma dimerus is a freshwater species that presents social hierarchies, biparental care and complex stereotyped breeding activities; thus it has become an interesting model for reproductive, behavioral and ecotoxicological studies. The aim of this study was to describe the effects of FLX on the reproductive axis and aggressive behavior during breeding of C. dimerus. Male and female fish were housed in individual aquaria and received injections of 2 µg/g FLX (i.p) every 48 h during 2 weeks. Estradiol, testosterone and cortisol levels were measured in plasma using ELISA. Gonadotropin levels were semi-quantified by Western blot from pituitary homogenates. Liver and gonads were processed for histology. Additionally, aggressive behavior was evaluated using the resident-intruder paradigm. Males of a reproductive pair were injected with saline or 2 µg/g FLX (i.p) daily for 3 days after spawning and subjected to the temporary presence of an intruder male. Aggressive interactions from the male and female of the reproductive pair towards the intruder were recorded and quantified. In males, FLX caused a reduction of plasma estradiol. In these individuals no estrogenic effect was evidenced since FLX failed to induce vitellogenin, a common endpoint for estrogenic disruption. Pituitary FSH and LH content remained unchanged in males; on the contrary, female FSH content was reduced significantly after treatment with FLX. Liver and gonad histology was normal in both males and females. In the behavioral study, both male and female increased aggressive interactions after spawning, regardless of FLX injection. However, of all the behavioral interactions studied, biting and tail beating behavior from males showed variation upon FLX injection, the former increasing and the latter decreasing. Since hatching induces changes in parental care behavior, it would be interesting to evaluate the reproductive axis and the aggressive pattern differentially during each reproductive stage.