DETECTION OF ARBUSCULAR MYCORRHIZAL FUNGI ASSOCIATED WITH PECAN (Carya illinoinensis) (Wangenh.) K. Koch) TREES BY MOLECULAR AND MORPHOLOGICAL APPROACHES

ROXANA COLOMBO; MARIANA PERGOLA,; ROXANA COLOMBO; MARIANA PERGOLA,; LAURA FERNANDEZ BIDONDO; MARINA RECCHI; LAURA FERNANDEZ BIDONDO; MARINA RECCHI; VANESA SILVANI; GODEAS ALICIA; VANESA SILVANI; GODEAS ALICIA

MycoKeys

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018 vol. 42 p. 73 - 88

1314-4057

Arbuscular mycorrhizal (AM) fungal community associated with pecan (Carya illinoinensis) roots andrhizospheric soils was assessed by spore isolation and morphological characterisation and by pyrosequencing of AM molecular markers. The AM fungal community associated with pecan growing in the field,was always more diverse than that associated with pecan growing in containers. This was not observedwhen AM richness was studied, suggesting that soil disturbance by a reduction in host plant richnessleads to a less equitable distribution of AM fungal species, in contrast to natural soils. The chosen primers(AMV4.5F/AMDGR) for pyrosequencing showed high AM fungal specificity. Based on 97% sequencesimilarity, 49 operational taxonomic units (MOTUs) were obtained and, amongst these, 41 MOTUscorresponded to the Glomeromycota phylum. The number of obtained AM sequences ranged from 2164,associated with field samples, to 5572 obtained from pecan trap pot culture samples, defining 30 and 29MOTUs, respectively. Richness estimated by conventional species identification was 6 and 9 AM fungalspecies in soil and pot samples, respectively. Claroideoglomus lamellosum, Funneliformis mosseae and Entrophospora infrequens were the only taxa detected using both techniques. Predominant sequences in thepecan rhizosphere samples, such as Rhizoglomus irregulare and other less abundant (Dominikia iranica,Dominikia indica, Sclerocystis sinuosa, Paraglomus laccatum), were detected only by pyrosequencing. Detection of AM fungal species based on spore morphology, in combination with molecular approaches,provides a more comprehensive estimate of fungal community composition