IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
artículos
Título:
Germinability and antioxidant metabolism in Passiflora caerulea L. seeds exposed to salt stress
Autor/es:
HUMBERTO FABIO CAUSIN; JOSÉ MARÍA CHAÍN
Revista:
Revista del Museo Argentino de Ciencias Naturales
Editorial:
Museo Argentino de Ciencias Naturales Bernardino Rivadavia
Referencias:
Año: 2018 vol. 20 p. 23 - 34
ISSN:
1514-5158
Resumen:
P. caerulea is the Passiflora species with the widest distribution in Argentina. Despite the need tocultivate it to decrease the extraction impact on its natural populations, the information on how environmentalfactors affect seed germinability is scarce. In order to evaluate the greminative response and the role of the antioxidantmetabolism against saline stress, freshly collected seeds were germinated at 26° C in the presence of 0(control), 30, 60 or 90 mM NaCl. The percentage of germination, the content of soluble and carbonylated proteins,the activity of antioxidant enzymes and the generation of reactive oxygen species both in the pre-germinationphase and during germination were evaluated. The removal of the aryl and the micropylar integument after 3 himbibition proved to induce a rapid germination and activation of the enzymes catalase, glutathione S-transferaseand peroxidases, together with an increase and a decrease in the generation of O2- and H2O2, respectively, at theroot apex. Even though the germination and activity of some of the studied enzymes decreased with the increasein external NaCl levels, when comparing populations of the control and 90 mM NaCl treatments having similargermination percentages, a marked induction of peroxidase activity was observed in the latter group. The proportionof carbonylated proteins did not differ among treatments, which suggests that, despite this species cannot beconsidered as halo-tolerant, the fact that the antioxidant metabolism efficiently contributed to prevent oxidativedamage to the proteome may constitute an important mechanism to facilitate its establishment in environmentswith moderate salinity.