TOXICITY, FREEZING AND COLD STORAGE TESTS OF NATIVE SPECIES SEMEN

LUCIANA ANTUNES DE MATTOS; HELAINE DOS REIS FLOR; TIAGO VIANA DA COSTA; LÓPEZ GRECO LS; LIDIA MIYAKO YOSHII OSHIRO; EMANUELA PAULA MELO

BOLETIM DO INSTITUTO DE PESCA

INST PESCA

Año: 2017 vol. 43 p. 334 - 346

0046-9939

In the first part of this study, toxicity tests were performed on the sperm of Macrobrachiumacanthurus using four cryoprotectants for periods of 10 and 20 min at concentrations of 10 and 20%.In the second part, cryopreservation was performed by applying the least toxic cryoprotectant, andtwo freezing methods were tested over 24 hours: automated (protocols A and B) and conventional(protocols C and D). Protocol A exhibited a cooling rate of 0.5°C min-1 from -6°C to -32°C; protocolB was similar to A except for the starting temperature, which was room temperature; whereasprotocols C and D exhibited a cooling rate of 2 and 10°C min-1, respectively. The third part ofthe study was conducted to assess the lifespan of the sperm when stored at 5°C, in which spermviability was evaluated by a semen smear with eosin-nigrosin. The least toxic cryoprotectants were10 and 20% glycerol, and 10% methanol, and the equilibrium time was 10 minutes. The optimalcooling rate was 2°C min-1 for 10% glycerol, which had a sperm survival rate of 21.8%. Cold storagefor up to 3 days is recommended, presenting a sperm survival rate of 35.3%.Key words: caridea; cryopreservation; cryoprotectants; glycerol; methanol; shrimp