Somatic Embryogenesis in Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae)

GUERRA MIGUEL PEDRO; NEUSA STEINERI; FELIPE DO NASCIMENTO VIEIRAI; SARA MALDONADOII; MIGUEL PEDRO GUERRAI,*; FRANCINE LUNARDI FARÍAS-SOARES ; MALDONADO SARA.

METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)

Springer Science+Business Media New York

Lugar: Nueva York; Año: 2016 vol. 1359 p. 439 - 450

1064-3745

This chapter deals with the features of somatic embryogenesis (SE) in Araucaria angustifolia , an endangered and native conifer from south Brazil. In this species SE includes the induction and proliferation of embryogenic cultures composed of pro-embryogenic masses (PEMs), which precede somatic embryos development. A. angustifolia SE model encompasses induction, proliferation, pre-maturation, and maturation steps. Double-staining with acetocarmine and Evan?s blue is useful to evaluate the embryonic somatic structures. In this chapter we describe A. angustifolia SE protocols and analyzes morphological features in the different SE developmental stages.