CYTOTOXICITY AND MODULATION OF IL-10 AND TNF-ALPHA PRODUCTION IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS, OF NANOPARTICLES WITH ARACHIS HYPOGAEA L. TEGUMENT EXTRACT

PORTELA M.A.; ALBRECHT C.; SABINI CAROLA; CODEMO CAMILA; LUNA M ALEJANDRA; CARABAJAL CLAUDIA; PERALTA MARIA ISABEL; PERALTA MARIANA; REYNOSO EUGENIA

Buenos Aires

Congreso; 14th Latin American and Caribbean Congress of Immunology. 4 al 8 de noviembre de 2024.; 2024

Latin American and Caribbean Association of Immunology, Argentinean Society of Immunology and Argentinean Association of Clinical Immunologists

In viral infections, cytokines production is deregulated, contributing to immunopathogenesis. Plant extracts could stimulate the immune system. These compounds are often degraded before consumption. Their incorporation into nanoparticles increases their intestinal absorption and bioavailability.The aim was to evaluate in vitro cytotoxicity and production of IL-10 and TNF-α of nanoparticles with Arachis hypogaea L. tegument ethanolic extract (TEE).Soy lecithin multilamellar vesicles with TEE were obtained and subjected to extrusion resulting in unilamellar vesicles with TEE (UVE). Then, they were run into a sephadex column to obtain purified unilamellar vesicles (PUV). Empty unilamellar vesicles (EUV) without TEE were produced.Cytotoxicity and immunomodulation studies were performed with peripheral blood mononuclear cells extracted from healthy humans (Banco de Sangre, UNC) using Ficoll-Hypaque. Cells were seeded in 96-well plates with RPMI medium supplemented and treated with UVE, PUV (50-200µg/mL of TEE) and EUV, for 48 hours. Cell viability was assessed by trypan blue exclusion. To assess cytokine production, PBMCs were stimulated with dengue virus serotype 2 (DENV2), and were treated with UVE, PUV (50µg/mL of TEE) and EUV (10%). Cell controls (CC) and positive control (VC), treated with DENV2 (50 PFU/well), were included. TNF-ⲁ and IL-10 production was assessed in supernatants by commercial ELISA kits.ANOVA, t-test, and nonlinear regression analysis were performed (GraphPadPrism 8.0).UVE, PUV, and EUV were not cytotoxic. TNF-α production was 168.5±45.25 and 131.1±4.71pg/mL for UVE and PUV, respectively with a significant difference to VC (498.1±64.58pg/mL). IL-10 production also showed significant differences between UVE (724.7±57.66pg/mL) and PUV (718.9±13.60pg/mL) and VC (1243±151.8pg/mL) . No significant differences were found for EUV in any case.These results demonstrate that nanoparticles with TEE could modulate immune response to exposure of viral infections at non-cytotoxic concentrations.