Basal frequencies of micronuclei in three fish species from coastal and freshwater ecosystems of Argentina

ITURBURU FERNANDO GASTÓN; MENONE MIRTA LUJÁN; LOMBARDERO LUCAS RODRIGO; PANZERI, ANA M.; CRUPKIN ANDREA CARINA

Asunción

Congreso; XI Congreso Latinoamericano de Mutagenesis, Carcinogenesis y Teratogenesis; 2019

Asociación Latinoamericana de Mutagénesis, Carcinogénesis y Teratogénesis Ambiental.

Aquatic ecosystems are impacted by different types of anthropogenic pressures. Hence, to evaluate health status of organisms in these habitats becomes important. The objective of the present work is to analyze the frequency of micronuclei (MN) and other nuclear abnormalities (NA) in different fish species representative of coastal and freshwater ecosystems. This work is included in a major project that attempts to evaluate in a temporal scale the anthropogenic pollution of Mar del Plata city coast and the Mar Chiquita coastal lagoon basin in Argentina. The following species were selected as biomonitors: Oligosarcus jenynsii (n=10) from Nahuel Ruca lagoon, Odontesthes argentinensis (n=7) from Mar Chiquita coastal lagoon, and Gobiosoma hemigymnum (n=8) from Mar del Plata's harbor. Blood samples were obtained in summer 2018 from mentioned species and blood smears were prepared, fixed in methanol and stained with Giemsa 15% for their observation by microscopy at 1000X. Obtained results showed basal frequencies (mean± s.d.) for O. jenynsii of 1.35± 0.58 and 6.75± 5.05, for O. argentinensis of 1.07± 1.54 and 5.29± 1.58, and for G. hemigymnum of 2.97± 1.49 and 149.81± 29.78 for MN and NA, respectively. These are the first data obtained from the first sampling period, and they will be completed after five years of project by analyzing correlations of these biomarkers of genotoxicity with physicochemical characteristics of the water as well as measurement of pollutants.