Fish Bol in Argentina

DÍAZ DE ASTARLOA, J. M.

Porto Seguro

Conferencia; 3rd International Fish Barcode World Conference; 2017

Sociedad Brasileira de Ictiologia

Argentina has an area of c.a. 2,8 M km2 and the largest continental shelf in the world, with nearly one million km ². The fish fauna, both freshwater and marine, comprises about 1000 species. In addition, about 150 species occur in the Argentine Antarctic Peninsula.Since 2006 the active participation of Argentina in the Global Fishbol campaign began. Several Research cruises have been carried out, building a large fish collection throughout the Argentine Sea including coastal waters, the continental shelf and slope. Between 2011 and 2014 Antarctic cruises have been added and since 2010 we began sampling in inland waters performing 18 fieldwork trips in 160 different sites.So far, the fish and tissue collection comprise almost 5000 specimens and about 520 species, representing c.a. 47% of the fish species reported for Argentina.Extraction and amplification were performed in two International Barcode of Life reference Laboratories in Argentina. Sequencing was performed in Advanced Analysis Center´s Genomics Facility and in the Canadian Centre for DNA Barcoding (University of Guelph, Ontario, Canada). The Barcode Index Number (BIN) was used to estimate the number of species directly from the barcode records and congruence of these estimates with the distance based and character based approaches were evaluated.The barcodes generated were used to increase the BOLD reference library and to test the immensely valuable tool of Barcoding for specific discrimination Argentinean fish. As a result several publications including description of new species and global studies of Barcoding application to marine and freshwater fishes have been produced.