IIMYC   23581
INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Biomarker responses elicited by a commercial formulation of Fipronil in a native species (Cyphocharax voga) after branchial ex vivo exposure.
Autor/es:
SANTILLAND DEIU A.; ONDARZA P.M.; MIGLIORANZA K.S.B.; DE LA TORRE F.
Lugar:
Buenos Aires
Reunión:
Congreso; 11 th Biennial Meeting Society of Environmental Toxicology and Chemistry Latinoamérica.; 2015
Institución organizadora:
Society of Environmental Toxicology and Chemistry Latinoamérica.
Resumen:
Fipronil is a current use pesticide whose toxicity is still unknown in many non-target organisms (eg, fishes). Gills are considered the first organ where insecticides impact in aquatic organisms, therefore gill biomarker responses could be indicative of the immediate damage produced in fish. In this context, the biomarker responses promoted by a commercial formulation of the insecticide fipronil (CLAP) were evaluated in juveniles of C. voga using a branchial ex vivo exposure model. After sacrificing the animals, gill arches of each individual were dissected and arches were grouped in pairs. A number was assigned to each pair and subsequently were randomly assigned to the following experimental treatments: fish saline solution (C); two nominal concentrations (10 and 500 µg/L) of a commercial formulation of fipronil dissolved in saline solution (C1 and C2, respectively). Gills remained submerged in the solutions under continuous agitation for 1 h at 25 °C in glass containers. After incubation period, enzymatic activities of catalase (CAT) and glutathione-S-transferase (GST) as well as the levels of lipid peroxidation by TBARS and antioxidant capacity against peroxyl (ACAP) were determined. Differences between groups were analyzed using one way ANOVA and Tukey´s multiple comparisons. Gill exposure to C1 promoted an increase of 90% in GST activity respect to C group, however this raise was not significant in C2. Regarding the CAT activity, only C2 group showed a significant decrease (54%) respect to C. In terms of oxidative damage, exposition to both C1 and C2 fipronil concentrations promoted an increase in TBARS levels (87 and 116%, respectively). In the same way, an important decrease in total antioxidant capacity was also verified after gill exposure in C1 (246%) and C2 (450%) fipronil media. These results indicate that the assayed fipronil formulation proved to be an inducer of a significant oxidative stress in gills. In addition, the sensibility of this native species in combination with this fast test constitutes a useful tool in evaluating toxicity of formulated insecticides.