Effects of Undaria pinnatifida polysaccharide extracts on antioxidant activity of the penaeoid shrimp.

FENUCCI, J.L.; ESPINO, M.L.; DÍAZ, A.C.; VELURTAS, S.M.

Seattle

Congreso; Aquaculture America 2014; 2014

WORLD AQUACULTURE SOCIETY

The basic metabolism of an aerobic organism involves the production of free radical (RL) and oxygen reactive species (ROS), which is controlled by antioxidant mechanisms enzymatic and nonenzymatic. However, a small proportion (2-3%) of RL and ROS can evade this protection, causing cell damage. The addition of antioxidants in the diet of these organisms can improve your overall physiological state. Recently, the marine algae extracts showed to have antioxidant properties, which are attributed to the presence of polysaccharides. The objective of the study was to determine the effects of U. pinnatifida extracts as dietary supplement on A. longinaris y P. muelleri, measuring the total antioxidant activity (AT). Shrimps were fed for 4 weeks with two diets supplemented with extracts of U. pinnatifida: 1g and 2g/100g diet (D1 and D2), and other without additive (Control). At the end of the trial, the hepatopancreas were isolated for analysis of AT, which was estimated on the basis of the scavenging activity of the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH). All treatments showed radical scavenging ability, indicating that organ has natural antioxidant activity. Extracts of P. muelleri exhibited higher radical scavenging capacity, the DPPH was quenching almost completely in 16', in contrast in A. longinaris takes 30'. The supplementation with U. pinnatifida increased the AT in A. longinaris, to 10' was detected 15; 10 and 2% of DPPH remaining in the control, D1 and D2, respectively (Fig. 1A). Fig. 1B shows the antioxidant capacity measured for P. muelleri to 3', no significant differences were found between D1 (57%) and control (46%); however addition of more extract in diet increased the AT (D2: 31% DPPH remaining). In conclusion, extract of U. pinnatifida is proposed as dietary supplement to stimulate the antioxidant defenses of A. longinaris and P. muelleri. Further studies will determine if the addition of higher concentrations of this additive, improve antioxidant defenses.