IIMYC   23581
INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Unidad Ejecutora - UE
artículos
Título:
Histological and histochemical study of the hepatopancreasof two estuarine crab species, Cyrtograpsus angulatus and Neohelice granulata (Grapsoidea, Varunidae): influence of environmental salinity
Autor/es:
LONGO MV; DÍAZ AO
Revista:
ZOOLOGICAL SCIENCE
Editorial:
ZOOLOGICAL SOC JAPAN
Referencias:
Año: 2015 vol. 32 p. 163 - 170
ISSN:
0289-0003
Resumen:
The aim of this study was to analyze the histology and the histochemical distribution of glycoproteins
(GPs) and lipids of the hepatopancreas of Cyrtograpsus angulatus and Neohelice granulata
acclimated to salinities of 10 psu (hyperregulation) and 35 psu (osmoconformation). Sections of the
hepatopancreas of adult male crabs were treated with hematoxylin-eosin; Masson trichrome; Alcian
Blue (pHs 2.8, 1.0, 0.5); Toluidine Blue (pHs 5.6, 4.2); periodic acid Schiff; Sudan Black and Red. At
salinity 35 psu, the hepatopancreas of both species exhibited typical histological features, whereas
at salinity 10 psu, detachment of the basal lamina, desquamated epithelium, disrupted brush border,
loss of intercellular cohesion, hypertrophied tubular lumen, and hemolymph infiltration between cells
were observed in some zones. Resorptive cells (R-cells) and vacuoles of blister-like cells (B-cells) of
both species show a higher glycogen content at 35 psu than at 10 psu. At lower salinities, the cytoplasm
of the different cell types evidence higher contents of carboxylated GPs in N. granulata and
of sulfated GPs in C. angulatus. At both salinities, and at the two pHs in N. granulata and at pH 5.6
in C. angulatus, the brush border, the vacuoles of B-cells and the peritrophic membrane show
metachromasia. R-cell vacuoles and the cytoplasm of all cell types ?except for the E-cells?at all
salinities in both species show abundant lipid droplets. The results of the present study contribute
significant data to the histophysiology of crustacean decapods, favoring the comprehension of the
complex adjustment mechanisms facing saline stress in euryhaline crabs.