Phylogeny, phylogeography and systematics of the American pea crab genus Calyptraeotheres Campos, 1990 inferred from molecular markers

EMILIANO H. OCAMPO; RAFAEL ROBLES; MARIANA TEROSSI; JESÚS D. NÚÑEZ; MAXIMILIANO CLEDÓN; FERNANDO L. MANTELATTO

ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2013 vol. 169 p. 27 - 42

0024-4082

We used mitochondrial cytochrome oxidase I (COI) and the large ribosomal subunit (16S) genes to establish evolutionary relationships among species of Calyptraeotheres, to evaluate their usefulness as DNA-barcoding genes, and to assess the molecular diversity at population level within C. garthi. Bayesian, maximum likelihood and maximum parsimony phylogenies confirmed the monophyly of Calyptraeotheres, showing that the ancestor of C. garthi, C. hernandezi, and C. granti radiated after the formation of the Panamanian isthmus. This finding contradicts the Austral/Tropical hypothesis previously proposed based on morphological data. The COI and 16S distance matrices supported separation of species as well as the genera, and corroborated that DNA barcoding is a useful tool and complements the classical taxonomy in Pinnotheridae. Phylogenetic and genetic distance analyses suggested that C. hernandezi is a junior synonym of C. garthi. Finally, C. garthi did not show a population structure across its distribution range, and showed a pattern consistent with a recent population expansion event that began 230-300 kybp.