LOW SALINITY EFFECT ON ALKALINE PHOSPHATASES IN PRE/POST-INGESTA HEPATOPANCREAS OF Neohelice granulata

PINONI, SA, LÓPEZ MAÑANES, AA

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2013 vol. 37 p. 11 - 11

0327-9545

Integrative studies on digestive adjustments at the biochemical level upon hyper-regulation conditions in euryhaline crabs are lacking. The aim was to determine the pre and post-ingesta effect of low salinity (S) on levamisole-insensitive (LI) and levamisole-sensitive (LS) alkaline phosphatase activities (AP) in hepatopancreas (HP) of N. granulata. Adult male crabs (Mar Chiquita lagoon, Bs. As. province) maintained for 14 days (feeding regime: 3 times a week) at 35 (osmoregulation) or 10 (hyper-regulation) ? S were unfed for 5 days (pre-ingesta=PrI) and then fed. AP were determined at PrI, and at short (2h=t2, 4h=t4) and long-term (12, 23, 46h) post-ingesta (PoI). Supernatant of 10000xg 15min from HP homogenate (0.1MTris-HCl pH 7.4) (4 ml bufferxg tissue-1) was used. LI and LS (µmol pNPxmin-1xmg prot-1) were measured by pNPP hydrolysis (9.5mM) in 0.1M Tris-HCl, 4mM MgSO4 (pH 8.5) with (LI) and without 16mM levamisole. LS: difference between both assays. LI and LS at PrI were higher at 10 (LI=682, LS=210) than in 35 (LI=31, LS=4) ?S (n=5, ANOVA, p