IIMYC   23581
INSTITUTO DE INVESTIGACIONES MARINAS Y COSTERAS
Unidad Ejecutora - UE
artículos
Título:
Osmoregulation and Na+K+ATPase activity in gills of Neohelice granulata from Bahía Blanca.
Autor/es:
PINONI, S.A., LÓPEZ MAÑANES, A.A.
Revista:
BIOCELL
Editorial:
INST HISTOL EMBRIOL-CONICET
Referencias:
Lugar: Mendoza; Año: 2012 vol. 32 p. 62 - 62
ISSN:
0327-9545
Resumen:
Although flexibility in osmoregulation is a key strategy for the occupancy and using of habitat in euryhaline crabs, studies on the role of branchial Na+K+ATPase activity (NKA) in hyporegulation and comparative intraspecific studies on osmoregulation pattern in individuals from different geographic areas are lacking. The aim of this work was to determine osmolality of the hemolymph (HL) and NKA in anterior 1-5 (AG) and posterior 6-8 (PG) gills in adult males of N. granulata from Bahía Blanca estuary exposed to 10, 35 and 45‰ salinity (S) to establish the occurrence of osmoregulatory flexibility, possible adjustments in NKA and comparative aspects with crabs from different areas. Osmolality (mOsmxkg-1) in HL and the medium (M) was determined with cryoscopic osmometer. 2500xg supernatants (homogenization medium: 0.25M sucrose/0.5mM EGTATris pH 7.4) were used as enzyme extracts. NKA (nmol Pixmin-1xmg prot-1) was assayed by measuring 5mM ATP hydrolysis in (mM): 20 imidazole /100 NaCl/30 KCl/0.5 EGTA, pH 7.4; control: without KCl, 1 ouabain. t-test was used as statistical analysis (p