Comunidad bacteriana ruminal en cabras alimentadas con alfalfa fresca.

GRILLI, D.; KOPECNY J; MRAZEK J; FLIEGEROVA K; PAEZ LAMA S; CERÓN M; SOSA MA; ARENAS N

Tucumán

Jornada; III Reunión Conjunta de Sociedades de Biología de la Argentina.; 2015

Sociedad de Biología de Tucumán

Ruminants fed with fresh alfalfa develop an alteration in the rumen called bloat. This condition begins when animals take fresh forage with a high concentration of soluble proteins. The aim of this study was to analyze the structure of rumen bacterial community, by quantitative PCR, from fistulated goats fed with fresh alfalfa. The animals were monitored for clinical signs of bloat, after the change of feeding. The samples were collected 4 days before the dietary change and subsequently according to the intensity of the signs. DNA extraction was performed by a method that combines mechanical cell lysis with the column filtration steps of DNA. The quantification of selected bacteria was performed with the MX3005P qPCR System using PCR primers targeting 16S rDNA gene fragments. The serially diluted DNA isolated from known numbers of cells of the reference strains was used as standard for the construction of a calibration curve, which allowed calculation of the number of copies of the gene. Mean total copies number of 16S rDNA gene was compared with ANOVA followed by Tukey´s HSD procedure. Bacteroidetes and Bacteroides-Prevotella group decreased (p