IANIGLA   20881
INSTITUTO ARGENTINO DE NIVOLOGIA, GLACIOLOGIA Y CIENCIAS AMBIENTALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Proteolytic digestion of human hair samples as an alternative to conventional digestion for analysis of PBDEs
Autor/es:
CAMARGO, ALEJANDRA B.; LOCATELLI, DANIELA; MAMMANA, SABRINA B.; ALTAMIRANO, JORGELINA C.
Lugar:
Birmingham
Reunión:
Conferencia; XI Network POPs Conference, University of Birmingham, UK; 2018
Resumen:
The Persistent Organic Pollutants (POPs) analysis has become a useful tool to assess the population´s exposure to the environment. Blood is considered an ideal matrix to analyse, because it is in contact with all the organs and tissues of the organism, but it has several limitations in its application: a) it is an invasive sample which causes a significant impact on the response of donors, b) difficult sampling in children. In this way, new biomarkers have been discussed, taking into account the need to use non-invasive matrices such as hair, nails, urine and saliva. Hair analysis has many advantages: easily available tissue, non-invasive sampling, sample collection, simple transport and storage, possibility of monitoring in the short and long term and, mainly, stable matrix with low or no active metabolic activities. Therefore, once the chemical is incorporated into the keratinous tissue, the levels remain relatively unchanged, which makes it an appropriate sample for the determination of trace compounds such as POPs.In the present work, a novel proteolytic digestion is proposed in samples of human hair suitable for the preparation of samples and subsequent chromatographic analysis. Likewise, it will allow the coupling of liquid-liquid microextraction techniques already studied by the group, such as DLLME. The technique of proteolytic digestion is proposed as an alternative to the classic acid/base digests used for hair, being effective, simple, fast and of low environmental impact, for the determination of POPs and subsequent analysis by gas chromatography with detection of electron capture ( GC-ECD). The proposed methodology enables the determination of POPs such as PBDEs, PCBs and OCPs in human hair samples using an environmentally friendly digestion, an alternative to conventional known digestions.By experimental design, the experimental variables that influence this phenomenon will be evaluated: concentration of the digesting agent, concentration of SDS, use or not of agitation and temperature and time of digestion, using a factorial design 2k-p for previewing the experiment.After studying and analysing the experimental variables of preliminary assays, it was observed that, in order to achieve optimal digestion of 10 mg of hair, 67 mM Tris-HCl buffer, 0.03 mg mL-1 digestion agent, 0.5% (p/v) of SDS with agitation at 600 rpm and 55 °C for 120 minutes.