INIBIOMA   20415
INSTITUTO DE INVESTIGACIONES EN BIODIVERSIDAD Y MEDIOAMBIENTE
Unidad Ejecutora - UE
artículos
Título:
Chemical effects on dye efflux activity in live zebrafish embryos and on zebrafish Abcb4 ATPase activity
Autor/es:
BIECZYNSKI, FLAVIA; SCHOLZ, STEFAN; BURKHARDT MEDICKE, KATHLEEN; LUCKENBACH, TILL; LUQUET, CARLOS M.
Revista:
FEBS LETTERS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2020
ISSN:
0014-5793
Resumen:
ATP-binding cassette (ABC) transporter proteins include efflux pumps that confer multixenobiotic resistance to zebrafish embryos, a valuable toxico/pharmacological model. Here, we established an automated microscopy-based rhodamine B dye accumulation assay in which enhanced dye accumulation in live zebrafish embryos indicates inhibition of multixenobiotic efflux transporter activity. Twenty structurally divergent known substrates and/or inhibitors of human ABC transporters and environmentally relevant compounds were examined using this assay and the ATPase activity of recombinant zebrafish Abcb4 as readouts. These two assays confirmed that Abcb4 functions as an efflux transporter in zebrafish, whereas they gave discordant results for some of the tested substances. The dye accumulation assay in zebrafish embryos could be useful to screen environmental pollutants and other chemicals for efflux transporter interaction in a medium-throughput fashion.