IFLP   13074
INSTITUTO DE FISICA LA PLATA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Magneto-targeting of Adipose-derived stem cells to improve tissue regeneration
Autor/es:
VANINA USACH; PAULA A. SOTO; MARCELA B. FERNÁNDEZ VAN RAAP; PIÑERO, GONZALO; SETTON-AVRUJ, PATRICIA
Lugar:
Caparica
Reunión:
Simposio; IV International Caparica Symposium on Nanoparticles, Nanomaterials and Applications 2020; 2020
Resumen:
Adipose-derived mesenchymal stem cells (AdMSC) are multipotent adult stem cells which arebeing fully investigated for regenerative therapies. Wallerian degeneration (WD) is an efficientanimal experimental model in mimicking the impact of peripheral nerve lesion to shed light onpossible regeneration strategies. AdMSC transplant is a useful tool for regenerative therapies,while magneto targeting is a nanotechnological strategy to mobilize magnetic nanoparticle (MNP).In this context, the aim of the present work was to test whether magneto targeting of AdMSC-loaded MNP (AdMSC-MNP) can reach specific tissue guided by an external magnetic field andenhance the regenerative ability of AdMSC upon rat sciatic nerve lesion.To test our hypothesis, AdMSC were characterized through immunocytochemistry, Western blotand flow cytometry for multipotent cell marker expression. MNP internalization to AdMSC wasevaluated through transmission electron microscopy and vibrating sample dc-magnetometry(VSM) experiments. Likewise, confocal microscopy and VSM analyses were performed to evaluatethe arrival of AdMSC-MNP at the injured nerve. Finally, cell transplantation effects on nerveregeneration were evaluated both in terms of morphology and conduction throughimmunofluorescence, Western blot and electrophysiological experiments.Our results show that AdMSC in passage 3 express multipotent markers CD105, CD90 and CD73but not CD11b or CD45, and can internalize 2 to 4 pg MNP/cell. We demonstrate AdMSC-MNP tosupersede AdMSC arrival exclusively at the lesion site and their beneficial effects on sciatic nerveregeneration.In short, our results prove that magneto targeting of AdMSC-MNP constitutes a novel and valuabletool to promote nerve regeneration by enhancing AdMSC arrival at the lesion site.