Imiging cell nanoparticle interaction

V. USACH; D. CORAL ; M. B. FERNÁNDEZ VAN RAAP; P. A. SOTO; P. SETTON-AVRUJ

La Pata

Workshop; Imaging Techniques for Biotechnology and Biomedical Applications Workshop; 2016

New diagnosis and therapeutics protocols involving the use of nanoactuators, nanobiodevices and nanoscience methodologies are of great interest nowadays. The main ingredient of these protocols is the interaction between biological soft matter and inorganic materials. Among inorganic materials iron oxide magnetic nanoparticles display a key role being used as carriers for drug, gene and cell delivery and as heat transducers in magnetic hyperthermia. Cells are the main nanoparticle receptors and the interaction among them is crucial, considering that the carriers must be able to reach its objective, to enter the cell and exert its effects without triggering citotoxicity. These interactions determine the uptake and nanoparticles localization and structuring inside the cell, which quantification and visualization are of great importance. Here we will discuss some experiments involving magnetic nanoparticles internalization in human lung adenocarcinoma cells for magnetic hyperthermia [1] and on multipotent adipose derived cells for stem cell magnetic targeting to improve the regeneration of injured peripheral nervous system [2]. [1] M. de Sousa et al. J. Phys. Chem. C, 2016, 120 (13), pp 7339?7348 doi: 10.1021/acs.jpcc.5b12330[2] V. Usach et al. Transplantation (in press) 2016 doi: 10.1097/TP.0000000000001478