IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
An HPLC method for soy isoflavone extracts quantification using internal standard methodology
Autor/es:
REARTES, NOEMÍ; FERRAYOLI, CARLOS; TURCO, MAURICIO; MARTINEZ MARCELA; MARÍA CECILIA PENCI; NASSETTA, MIRTHA
Lugar:
Lisboa
Reunión:
Conferencia; 13th International Food Data Conference IFDC 2019; 2019
Institución organizadora:
INFOODS
Resumen:
The increasing interest in functional food products that offering health benefits, helping decrease or prevent the risk of diseases is evident. The isoflavone family belongs to the group of polyphenols, known as powerful antioxidants. Soybean and most of its by-products are rich in isoflavones as derivatives of the aglycones genistein, daidzein and glycitein. It is important to advance in the development of methods and processes to obtain extracts of isoflavones useful in the formulation of functional foods, dietary supplements and cosmetics. The purpose of this work was the development of an HPLC chromatography method including an internal standard (IS, caffeic acid) to improve isoflavones quantification. The identification of mother solutions both internal standard and each of the standards and of the mixtures of isoflavones corresponding to each point of the calibration curve was carried out in a Waters HPLC, Equipped with a UV-Visible detector. The separation was achieved with a ZORBAX Eclipse XDB-C18 column (4.6 x 250 mm, 5 μm), maintained at 40 ° C during the running time. The mobile phase consisted of a 1% solution of acetic acid in water (Solvent A) and a 1% solution of acetic acid in acetonitrile (Solvent C). The spectra were readed between 230 and 280 nm with a UV-Visible detector and the compounds were detected at 254 nm. The glycosides and aglycones of each family of isoflavones, as well as IS were identified by comparing their retention times and UV spectrum with commercial standards. Standards were evaluated daily for system quality assurance. The limit of detection (LOD) of Daidzin was 0,0230 µg/mL, for Glycitin 0,0608 µg/mL, for Genistin 0,0270 µg/mL, for Daidzein 0,1315 µg/mL and for Genistein 0,0017 ug/mL. The limit of quantification (LOQ) Daidzin was 0,0768 µg/mL, for Glycitin 0,2027 µg/mL, for Genistein 0,0900 µg/mL, for Daidzein 0,4383 µg/mL and for Genistein 0,0057 µg/mL. This work pretends provide a method to perform routinely analysis of soy isoflavone extracts from different matrices using conventional detectors for HPLC.