IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Natural anthraquinones as new photodynamic sensitizers with antimicrobial potentiality
Autor/es:
DIMMER JESICA; NUÑEZ MONTOYA SUSANA; MUGAS MARÍA LAURA; MARIONI JULIANA; CABRERA JOSE LUIS
Lugar:
Florida
Reunión:
Congreso; 2018 Biennial Meeting American Society for Photobiology; 2018
Institución organizadora:
American Society for photobiology
Resumen:
The world health organization strongly promotes the research in plants to obtain new therapeutic agents. In this framework, we initiated a research line to obtain photosensitizing compounds from nature, since the photosensitizers have shownto be highly active against microorganisms, especially when the photosensitization process is involved. Thus, they are projected as possible drugs to be used in antimicrobial photodynamic therapy (APDT).We began with the chemical study of a plant popularly known for its phototoxicity, Heterophyllaea pustulata Hook f. (Rubiaceae), which grows in Argentina (Northwest) and Bolivia.It was established that its photosensitizing components were anthraquinone derivatives, isolating ten aglycon anthraquinones (AQs), three of which were found for the first time in nature (heterophylline, pustuline and bisoranjidiol). We continued with the chemical study of the othervegetal specie that belongs to this genus, H. lyciodes, obtaining seven AQs of which three were identified as new structures (5-chlorosoranjidiol, 7-chlorobisoranjidiol and lycionine). From lichens of the genus Teloschistes, parietin was isolated, a 1,8-dihydroxy AQ.After studying the photophysical and photochemical properties of each AQ, we evaluated its photodynamic effect over microorganisms and viruses. First, cytotoxicity was determined on a mammalianeukaryotic cell line (Vero cells), establishing a range of concentrations with low or no toxicity, which were subsequently used to evaluate the antimicrobial effects. We have shown that some of these AQs exhibit a significant photoinducedeffect in vitro against Herpes Simplex Virus type 1, Candida spp., Leishmania amazonensis and different bacterial strains. Also, the photosensitizing mechanism was investigated for AQs in Candida tropicalis biofilm. Results project these AQs as new photodynamic sensitizers with potential use in APDT.