IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Oxidative damage as a toxicity mechanism of silver bionanoparticles in human trophoblasts
Autor/es:
GOMEZ DS; PÁEZ PL.; QUINTEROS M ; GUIÑAZÚ N; BUSTOS PS; ORTEGA MG;
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Conjunta SAIC SAI SAFIS 2018; 2018
Resumen:
Within the nanomaterials, the metal nanoparticles have gained agreat popularity due to their potential antimicrobial activity. Silvernanoparticles (AgNPs) biosynthesized by metal-reducing culturesupernatant of Pseudomonas aeruginosa, have demonstrated animportant antibacterial activity. Recently we showed that AgNPs arecytotoxic for human trophoblasts. The aim of this study was to deep-en the mechanism of toxicity of these nanoparticles in the humantrophoblast.HTR8/SVneo cell line was exposed for 6 and 24 h at different con-centrations of AgNPs (0.3-1.5 pM). Cell viability, ROS productionand endogenous defenses (glutathione content (GSH), catalase(CAT), superoxide dismutase (SOD) and glutathione s-transferase(GST) activities) were determined. Biomarkers of macromoleculesoxidative damage were evaluated, for protein oxidation (AOPPmethod) and genotoxic damage (comet alkaline assay).The exposure of HTR8/SVneo cells to AgNPs produced a decreasein cell viability (CI50 for 6h and 24 h were 1.21 pM and 0.81 pMrespectively). All the AgNPs concentrations evaluated induced anincrease in ROS production and GSH content. The antioxidant en-zymes SOD and CAT increased the activity at the highest concen-trations assayed (1.5 pM at 6 h and 0.75-1.5 pM at 24 h). While GSTactivity, a detoxifying enzyme, decreased after AgNPs treatment.Regarding oxidative damage to biomolecules an increase in proteinoxidation and genotoxic damage were observed in cells exposed toAgNPs at the highest concentrations assayed. To elucidate wheth-er oxidative stress is a toxicity mechanism triggered by AgNPs tox-icity trophoblast cells were preincubated with the antioxidant NAC(N-acetylcysteine, 2 mM). The treatment with NAC reverted celldeath, protein oxidation and genotoxic damage.Therefore, the AgNPs biosynthesized by P. aeruginosa are cytotoxicto human trophoblast cells and oxidative imbalance would be thetoxicity mechanism involved in cell death and macromolecule dam-age triggered by these nanoparticles.