ALLANTOIN MODULATES ROS PRODUCTION IN ARABIDOPSIS THALIANA

BOGGINO, MF; DESIMONE M; TESSI, TM; LESCANO, CI; GONZALEZ, CA

Congreso; XVI CONGRESO LATINOAMERICANO DE FISIOLOGIA VEGETAL; 2018

In plants, several abiotic stresses lead to the overpro- duction of reactive oxygen species (ROS) which cause oxidative stress in cells. However, the functions of ROS have been largely discussed, being also implicated in the signaling of developmental processes and plant responses to stress.Allantoin, a purine-derived metabolite, is accumulated in plants exposed to different abiotic stresses and has been related with stress tolerance. The Ureide Per- mease 5 (AtUPS5) has been proposed as a mediator for its long distance transport. Interestingly, ups5mutant seedlings were sensitive to salt suggesting that allan- toin transport mediated by AtUPS5 is an important event for salt stress alleviation.To determine if AtUPS5-mediated allantoin long dis- tance transport could be also involved in ROS levels re- gulation, WT plants and ups5mutants were incubated with exogenous allantoin during 3 and 24 hours and after that ROS levels were determined. Allantoin con- tent increased in WT shoots, but not in ups5 ones. After 3 hours of allantoin treatment, ups5 mutants decrea- sed its O2·- levels compared to WT. However, 24 hours allantoin treatment led to a higher ROS production in the shoots of both genotypes. Our results suggest a role for allantoin transport in modulating ROS production.