IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Arabidopsis thaliana Ureide Permeases 5 (AtUPS5) connects compartments involved in ureide metabolism
Autor/es:
MARTINI, C; SCHUMACHER K; BOGGINO, MF; GONZALEZ, CA; LESCANO, CI; TESSI, TM; DESIMONE M
Reunión:
Congreso; XVI CONGRESO LATINOAMERICANO DE FISIOLOGIA VEGETAL; 2018
Institución organizadora:
SAFV
Resumen:
The transport of metabolites plays an important role for optimal plant adaptation to the environment. Allan- toin is a purine metabolite that was recently related with stress tolerance in plants. The subcellular locali- zation of enzymes that lead to allantoin synthesis sug- gest that allantoin is produced in peroxysomes and loaded to vesicles for export via the secretory pathway, although the transport system remains still unidenti- fied. AtUPS5 (Arabidopsis thaliana Ureide Permease 5) is a transmembrane protein that transports allantoin with high affinity. We studied the subcellular fate of splicing variants AtUPS5L (Large) and AtUPS5s (short) by tagging them with fluorescent proteins in their cen- tral loops. The capability of these fusion proteins to co- rrectly complement the function of native ones was proved by nutritional and salt stress experiments. Both variants were localized in the endoplasmic reticulum, but the AtUPS5L variant was also localized in the trans- Golgi network/early endosome (TGN/EE). The subcellu- lar fate of AtUPS5 splicing variants suggest that they could have different roles in allantoin distribution bet- ween subcelular compartments in the plant cells.