IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
FIRST MYCOVIRUS IDENTIFIED IN FUSARIUM VERTICILLIOIDES AND F. ANDIYAZI
Autor/es:
THEUMER MG; GARCÍA-PEDRAJAS MD; CAÑIZARES C; DAMBOLENA JS; JACQUAT A; IGLESIAS J
Lugar:
Ciudad Autónoma de Buenos Aires
Reunión:
Jornada; XX Jornadas Anuales de la Sociedad Argentina de Biología (SAB) conjunto con XVII Jornadas de la Sociedad Uruguaya de Biociencias (sub) y segundas Jornadas Rioplatenses de Biología.; 2018
Institución organizadora:
SOCIEDAD ARGENTINA DE BIOLOGIA
Resumen:
The fungal pathogen Fusarium verticillioides (Fv) is the main cause of ear rot of corn in Argentina and one of the major producers of the mycotoxin, fumonisins, which can cause health problems to humans and farm animals. The use of synthetic fungicides is the most used strategy to control the phytopathogenic fungi in plants. However, in view of the potential negative impact on the environment, new friendlier strategies such as biological control should be taken into account. The mycoviruses are intracellular infectious agents specific to fungal cells. These generally have double-stranded RNA genomes. Although it has not been reported that most of mycoviruses cause phenotypic changes in the host, some of them have been associated to phenotypic alterations including hypovirulence in phytopathogenic fungi. Due to this, the mycoviruses could be promising biocontrol agents to phytopathogenic fungi. Until today, there are no reference about mycoviruses isolated from Fv. The objective of this study was to determine the incidence of the mycoviruses in Fusarium spp. strains isolated from Argentina. One hundred monosporic isolates of Fusarium spp. were isolated from maize and sorghum in Argentina. The presence of dsRNA was evaluated using CF-11 cellulose chromatography. The obtained results demonstrated that among 100 isolates analyzed, 2 of them showed a monopartite dsRNA extrachormosomal bands (approximately 3.5 Kbp), after electrophoresis in 0.8% agarose. These genetic elements were vertically transmitted during conidiation, and showed resistance to S1 nuclease and DNAseI degradation. The fungal host strains were molecularly identified as Fusarium verticillioides and F. andiyazi, according to sequence homology of the tubulin and transcription factor 1-alpha genes. These monopartite dsRNA extrachormosomal fragments were named as dsRNA-Sec505 y dsRNA-162, according to the fungal isolate from which they were identified. The monopartite dsRNA fragments found in this work are consistent with the mycoviruses previously described in other fusarium species, so this is probably the first report of mycoviruses isolated in F. verticillioides and F. adiyazi until date. Sequencing studies are being performed in order to identify and classify into taxonomic groups, the detected mycoviruses. The potential of these mycoviruses to be used as a strategy to control the phytopathogenesis and mycotoxigenism of F. verticillioides and F. andiyazi, remains to be studied.