IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
NANOPARTICLES FOR CARIES LESION INFILTRATION AND POTENTIAL IN SITU REPAIRMENT
Autor/es:
ANGEL VILLEGAS N; BECERRA M C; MARTIN SAINZ AJA; SILVERO C., M. JAZMÍN; MOLINA, GUSTAVO; ROCCA D; PALMA S
Lugar:
bs as
Reunión:
Congreso; Reunion conjunta de sociedades de biociencias; 2017
Resumen:
Caries disease produces serious dental damage that eventually evolves in a tooth cavity. The aim of this work was to study the application of nanomaterials to infiltrate decayed tissue and promote a biological repairment response. Zinc nanoparticles (ZnO@NP, 50nm) and gold nanoparticles synthesized and stabilized with an antibiotic (amoxicillin@AuNP, 40nm) were selected for their known antimicrobial capacity and biocompatibility. ZnO@NP produced stable suspensions during 72h in: distilled H2O, PBS (pH=7.4), Thioglycolate broth, DMEM and IconR (a resin-based material mainly composed of tri-ethylene glycol dimethacrylate with high infiltration ability). The amoxicillin@AuNP stability, followed spectrophotometrically, was acceptable in DMEM and Thioglycolate broth (12.5%) for 72h, and was excellent in the resin until 72h. Agregation was noted immediately when mixing the two nanomaterials in brain-heart broth and in H2O2 (3%), which were alternatives for the culture of anaerobic bacteria and for infiltration, respectively. The interaction between nanomaterials and the demineralized dental surface was analyzed by SEM (Surface Electron Microscope) with coupled EDS (Energy Dispersive X-Ray Spectrometer). The load of nanomaterial was 22 times higher (Wt%: 42.9 ± 1) in dental samples treated with ZnO@NP carried in resin, than in those treated with ZnO@NP at the same concentration in PBS (Wt%: 1.9 ± 1). SEM images with mapping also showed that amoxicillin AuNP (1.5 μg/mL in PBS 50%) were attached on the dental surface, preferably in the demineralized area. Overall, best results were obtained by employing the resin as a carrier. This adhesive system based on methacrylates improved the nanomaterial adherence and recovered the dental structure by filling the porosities of the simulated lesion. Currently, their infiltration power and their effect on caries producing bacterial strains are being investigated.