Dandelion: potential antitumor agent against cervical cáncer

VENEZUELA F; MOSMANN J; MUGAS L; NÚÑEZ MONTOYA SC; MONETTI M; CUFFINI C; KIGUEN X; KONIGHEIM BS

Ciudad del Cabo

Congreso; 31ST INTERNATIONAL PAPILLOMAVIRUS CONFERENCE; 2017

International Papillomavirus Society

OBJECTIVE: To investigate the in vitro cytotoxicity and antiproliferative actions of extracts and latex of Taraxacum officinale in cervical cancer cell lines.METHODS: Ethanolic extracts were obtained from aerial parts and roots: Ap-EtOH and R-EtOH, whereas latex (L) was directly obtained from the roots. The dried extracts and latex were dissolved in DMSO (100 mg/mL). The study was conducted on cervical cancer cell lines (Caski and Hela) and normal keratinocyte (HaCaT). All cell lines were incubated with 15 different concentrations (10-1000 µg/ml) in triplicate, of each extract and latex. After 72 h incubation, cytotoxicity was assessed using the MTT dye. Cell morphology was observed using an inverted fluorescence microscope and apoptotic morphological changes were detected by Hoechst staining. Clonogenic assay was used to check ability of cell to grow and form colony.RESULTS: Both extracts and latex showed a dose-dependent cytotoxic effect in all cell lines. The order of cytotoxicity was R-EtOH> L> Ap-EtOH.R-EtOH and L were more cytotoxic in Hela and Caski that in HaCaT. The viability reduction was dependent on exposure time for R-EtOH and L. Fluorescence microscopy showed apoptotic morphological changes, such as fragmentation and chromatin condensation, in treated Hela and Caski cells. In clonogenic assay, different concentrations of R-EtOH and L decreased the number and size of colonies as compared to control in all cellsCONCLUSIONS: Our results suggest that T. officinale contains components that could induce apoptosis in Hela and Caski cells. Thus, it should be explored for its antitumor potential in cervical cancer.