IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Biosynthesized silver nanoparticles: decoding their mechanism of action in S. aureus and E. coli.
Autor/es:
CANO ARISTIZÁBAL V; QUINTEROS MELISA DE LOS ANGELES; PÁEZ PL; M.G. PARAJE
Lugar:
paris
Reunión:
Conferencia; 9th Euro-Global Summit on Toxicology and Applied Pharmacology; 2017
Resumen:
The generation of oxidative stress in bacteria by the presence of silver nanoparticles (AgNPs) is already widely known1,2. If the cell cannot respond to oxidative injury produced by increased species reactive oxygen (ROS), the oxidation of macromolecules such as proteins, lipids and DNA occurs, leading to the death of the bacterium3,4. In previous results (Quinteros et al.5), we observed as biosynthesized AgNPs that had antibacterial activity, generated an increase of ROS and RNI in Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, being responsible for their toxicity and bacterial death. According to the above, we set out to delve into the mechanism of action of AgNPs, by determining markers of oxidative stress, such as protein oxidation, lipids, DNA and changes in membrane potential in two reference strains S. aureus ATCC 29213 and E. coli ATCC 25922. We found that the increase in the levels of ROS is associated with the oxidation of different macromolecules important for the normal functioning of the cell, so that oxidative stress would be one of the mechanisms by which the AgNPs would exert their toxicity in these two strains of great clinical relevance. In this way, we are making a great contribution on the toxicity produced by AgNPs.Image Figure 1: Damage to proteins, lipids and DNA produced by ROS generated by AgNPs.Recent Publications (minimum 5)1.Wang C, Kim YJ, Singh P, Mathiyalagan R, Jin Y, Yang DC (2016) Green synthesis of silver nanoparticles by Bacillus methylotrophicus, and their antimicrobialactivity. Artif Cells Nanomed Biotechnol.44 (4):1127-32..2.Quinteros MA, Aiassa Martínez IM, Dalmasso PR, Páez PL. (2016) Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents. Int J Biomater.59710473.Páez PL, Becerra MC and Albesa I(2011) Comparison of macromolecular oxidation by reactive oxygen species in three bacterial genera exposed to different antibiotics. Cell Biochem Biophys 61(3): 467-72.4.Durán N, Durán M, Bispo de Jesus M.,Seabra D.,Fávaro W., Nakazato G. (2016)Silver nanoparticles: A new view on mechanistic aspects on antimicrobial activity. Nanomedicine: Nanotechnology, Biology, and Medicine 12:789?799.5.Quinteros MA, Cano Aristizábal V, Dalmasso PR, Paraje MG, Páez PL.(2016) Oxidative stress generation of silver nanoparticles in three bacterial genera and its relationship with the antimicrobial activity. Toxicol In Vitro. 2016 Oct;36:216-23