SSR markers assessed for peanut smut disease resistance.

BRESSANO, M.; DE BLAS, F.; SCHEFFLER, B; ARIAS, R.S.

Córdoba

Congreso; ADVANCES IN ARACHIS TROUHGT GENOMICS & BIOTECHNOLOGY; 2017

IBONE-CONICET-UNNE, GOB. DE LA PCIA. DE CBA., FUNDACIÓN MANÍ ARGENTINO

Peanut smut disease, caused by Thecaphora frezii(Carranza & Lindquist), can result in yield losseshigher than 50%. Several strategies have beendeveloped for disease management but they are stillinsufficient. The smut genetic resistance found inwild species and in Bolivian landraces is currentlythe best-known alternative to manage theepiphytotic, and SSR markers are a useful tool tofacilitate the rapid introgression of these attributesfor cultivar development. Three crosses betweenArgentinian susceptible cultivars and tworesistant/tolerant Bolivian landraces generatedsegregant populations. Also, three wild specieswere used to construct a fertile amphidiploidresistant to the peanut smut, which crossed with anelite line originated a population of 93 recombinantinbred lines (RILs). The parents of all thesepopulations were screened with 380 SSR markers?288 novel SSRs, 12 InDels and 80 SSRs obtainedfrom the literature?which were also analysed byBLAST2GO to discriminate those associated tocandidate genomic coding regions. An average of100 polymorphic markers across resistant andsusceptible parental materials were found, whichare being further tested on the four differentsegregant progenies to check for resistant attributeassociation.