Naturally photosensitizers in photodynamic therapy applied colorectal carcinoma cells using a three dimensional model

GILARDI P.,; CABRERA J.L.,; COGNO I., ; MONTOYA S., ; COMINI L., ; RIVAROLA V.

Mar del Plata.

Congreso; SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC), LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) Y LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE); 2016

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC), LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) Y LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL (SAFE)

The history of ethnobotanical studies shows that several genera of plants belonging to Argentina can be selected according to their use in traditional medicine as sources of cancer drugs. In our laboratory we have studied photodynamic capacity of different plant compounds called anthraquinones (AQs) in breast cancer cells. These compounds were isolated from a plant called Heterophyllaea pustulata HooK f. (Rubiaceae) growing northwest of Argentina. Photodynamic therapy (PDT) against cancer is the cytotoxic effect induced by the combined action of light and photosensitizing compounds (Fs). Cases have been reported eradication of colorectal cancer (CRC) using PDT as a treatment, but other data show an incomplete response to treatment, presenting long-term recurrence. The aim of this work was to study the effect of photodynamic AQs, such as Rubiadina and Soranjidiol in colorectal carcinoma cells in a three dimensional model. To do this, first the cytotoxic effect (MTT)of these molecules in a dimensional model cell cancer SW 480 colon was standardized and then nuclear morphology was evaluated using Hoescht staining (fluorescence microscopy). Both molecules with 50um and 15 minutes incubation did not result in decreased cell survival in dark conditions, but induced 80% cell death under irradiation (5J / cm2). At 16h post-treatment, a high number of apoptotic nuclei AQs evaluated for both observed. Research conducted in three-dimensional cultures currently represents a good option to emulate the situation of in vivo tissues. In our study we develop a three dimensional model of spheroids, which showed que 100uM and 1h incubation with 60% rubiadina death occurred with a lightdose of 20J/ cm2. However 60% of death 200um, 1h incubation and 30J / cm2 was observed for soranjidiol. The results encouraged us to further investigate in more detail the mechanism of action of these molecules for use as photosensitizers future to improve the efficacy of PDT for application in CCR.