IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EVALUATION OF THE ANTIMICROBIAL ACTIVITY OF A NEW PRESERVATIVE AGENT FOR CONTACT LENSES
Autor/es:
MARTINEZ SR; BECERRA M C; RAVETTI S; HERGERT LY; LIRIO M
Lugar:
Rosario
Reunión:
Otro; IV Reunion Internacional de Ciencias Farmaceuticas; 2016
Institución organizadora:
UNC, UNR
Resumen:
EVALUATION OF THE ANTIMICROBIAL ACTIVITY OF A NEW PRESERVATIVE AGENT FOR CONTACT LENSESMartínez S.1; Ravetti S.2; Lirio M.3; Becerra MC.1; Hergert L.3 E-mail: lisandrohergert@gmail.com1Depto. de Farmacia - IMBIV-CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba. Córdoba, Argentina; 2Centro de Investigaciones y Transferencia de la Universidad Nacional de Villa María (CIT-UNVM), Villa María, Argentina; 3Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas, Universidad Nacional de Villa María, Villa María, Argentina.Melaleuca alternifolia (Maiden & Betche) Cheel (family Myrtaceae) is harvested from natural stands and plantations for production of tea-tree oil. The therapeutic properties of essential oil of tea tree (AAT) are antimicrobial, antiseptic and antiviral.1,2 The AAT is colorless to pale yellow liquid and it is extracted from the leaves and twigs by steam distillation. Essential oils are mixtures of volatile organic compounds, mostly terpenes. The major component of AAT is monoterpene alcohol, terpinene-4-ol (30%). The aim of the study was to evaluate the antimicrobial activity of a new preservative agent for contact lenses obtained with AAT. Studies included evaluation of the effectiveness of a new preservative solution designed on planktonic and biofilms cultures of Staphylococcus aureus ATCC 29213 and Pseudomonas aeruginosa ATCC 27853.A new preservative solution was formulated containing AAT, edatato disodium, sodium chloride, monobasic and dibasic sodium and purified water. The cutoff point of the minimum inhibitory concentration (MIC) was determined in the first tube in which no turbidity was observed. The MIC for both strains were 0.5% V/V. Related to the biofilms eradication, the AAT extract decreased the number of viable colony forming units by 8 logarithmic units below the untreated samples. Also, the stability of the contact lens was checked by IR spectroscopic studies. The formulation containing AAT demonstrated good antimicrobial profile and it could be used as a potential agent to preserve contact lens. 1Mickienė R1, Bakutis B, Baliukonienė V. Antimicrobial activity of two essential oils. Ann Agric Environ Med. 18(1) (2011) 139-144.2Homeyer DC, Sanchez CJ, Mende K, Beckius ML, Murray CK, Wenke JC, Akers KS. In vitro activity of Melaleuca alternifolia (tea tree) oil on filamentous fungi and toxicity to human cells. Med Mycol. 53(3) (2015) 285-294.