Effect of irradiation frequency in photodynamic inactivation of Candida tropicalis biofilms by natural anthraquinones in combination with antifungals

JULIANA MARIONI, JOSÉ L. CABRERA, MARÍA G. PARAJE, SUSANA C. NÚÑEZ MONTOYA.

Mar del Plata

Congreso; LXI Reunión Científica de la Sociedad Argentina de Biología (SAIC); LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI) y XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental (SAFE).; 2016

A newstrategy to improve the effect of antifungal agents against biofilms is theircombination with natural compounds1. Weproved that rubiadin (AQ1) andrubiadin1-methyl ether (AQ2),two natural photosensitizing anthraquinones(AQs), reduce the biofilm formation of C. tropicalisdueto a photodynamic action2. Antibiofilm effect of each AQ was enhanced byapplying an irradiation sequence. Thus, the bioactive concentration of each AQwas halved, achieving also an increase in the reducing effect only in the caseof AQ2(63± 7 %R)3.Theaim of this work was to expand the antibiofilmactivity of these AQs against C. tropicalis,applying the same irradiation sequence but using each AQ combined each other orwith amphotericin B (AmB).