IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
capítulos de libros
Título:
Genomic characterization of the chili peppers (Capsicum, Solanaceae) germplasm by classical and molecular cytogenetics
Autor/es:
MOSCONE, E. A.; SCALDAFERRO, M. A.; GRABIELE, M.; ROMERO, M. V.; DEBAT, H. J.; SEIJO, J. G.; ACOSTA, M. C.; DAVIÑA J. R.; BARBOZA, G. E.; DUCASSE, D. A
Libro:
Physical mapping technologies for the identification and characterization of mutated genes contributing to crop quality
Editorial:
International Atomic Energy Agency (United Nations Organization)
Referencias:
Lugar: Viena (Austria); Año: 2009;
Resumen:
In the framework of the IAEA coordinated research project entitled “Physical mapping technologies for the identification and characterization of mutated genes contributing to crop quality” it was faced a genomic characterization of wild and cultivated samples of chili peppers (genus Capsicum) by classical chromosome staining methods (AgNOR and fluorescent chromosome banding) and fluorescent in situ hybridization (FISH). For the first approach, fluorochromes with affinity for specific chromosome regions were used, i.e., chromomycin A3 (CMA) and diamidino-phenyl-indole (DAPI) which have preference for GC-rich and AT-rich regions, respectively. In addition, Ag-staining to detect active nucleolus organizing regions was applied. Thus, heterochromatin could be characterized in respect to type, amount and distribution in the different accessions examined. On the other hand, the number and positon of active NORs could be determined. In respect to FISH, different DNA probes were used in order to map specific sequences in the chromosomes, i.e., 45S and 5S rDNA, telomeric sequences and cloned restriction fragments of repetitive nature. As an example of the work done here are presented the results obtained in a sample of Capsicum annuum var. annum (cultivar NMCA 10272), the most broadly exploited chili pepper. It should be mentioned that the present research is part of a network established in chili peppers with research groups of different countries in order to improve our knowledge in an important plant group utilized by man since long ago as chili peppers are included among the first cultivated plants in the history of humanity. The obtained results allowed to characterize Capsicum species and accessions. Furthermore, the possible evolutive pathways for chili peppers were developed on the bases of the available cytogenetic data.