IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Experimental poisoning by Heterophyllaea pustulata Hook. f. (Rubiaceae) in goats. Clinical, biochemical and toxicological aspects
Autor/es:
LAURA S. AGUIRRE; JOSÉ LUIS CABRERA; EDUARDO JUAN GIMENO; JUAN FRANCISCO MICHELOUD; MARIA LAURA MUGAS; SILVINA CAROLA GALLARDO; JULIANA MARIONI; OLGA GLADYS MARTINEZ; SUSANA CAROLINA NÚÑEZ-MONTOYA
Revista:
TOXICON
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2019 vol. 161 p. 56 - 61
ISSN:
0041-0101
Resumen:
The aim of this study was to investigate the clinical, biochemical and toxicological findings of the experimentallypoisoning induced by Heterophyllaea pustulata in goats. Ten healthy adult female Saanen breed goats were usedin the experiment. The goats were randomly assigned to two groups of five individuals: control and experimentalgroup (CG and EG). Both groups were kept in the same enclosure devoid of shade for 8 h daily. The EG receivedonly H. pustulata samples (leaves and thin steam) and water ad libitum. The CG received lucerne hay. Bloodsamples were taken at different times after oral administration of vegetal samples, and level of hepatic enzymes,total bilirubin, conjugated and non-conjugated bilirubin was measured, together with the detection of anthraquinones(AQs) and phylloerythrin by High Performance Liquid Chromatography with Diode-Array Detector andMass Spectrometry with Electron Spray Ionization and Quadrupole Time Of Fly analysis. At the same time, skinbiopsy samples were collected for AQs determinations. For histopathological examination, hepatic biopsysamples were collected on day 8. Clinically, all goats of the EG revealed photophobia, dermatitis and photosensitization.None of these goats developed jaundice or died during the experiment (15 days). In addition,affected goats exhibited a significant elevation in the serum levels of glutamic oxaloacetic transaminase, directbilirubin, and total bilirubin. Microscopic examination of the liver samples revealed slight degenerative lesions.Although phylloerythrin was not detected in sera, a high level of two predominant AQs in H. pustulata (rubiadin/soranjidiol) were noted between 24 and 72 h after plant consumption, which coincided with the period in whichthe clinical signs were more obvious. Since those AQs were not identified in skin samples, the clinical findingswere supported by the presence of AQs in sera. Finally, toxicological studies of the AQs are important, sincemany current works suggest their potential use in the photodynamic therapy.