IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Oxidative and nitrosative stress in Staphylococcus aureus biofilm
Autor/es:
ARCE MIRANDA JE, SOTOMAYOR CE, ALBESA I AND PARAJE MG.
Revista:
FEMS MICROBIOLOGY LETTERS
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2011 p. 23 - 29
ISSN:
0378-1097
Resumen:
SUMMARYDiverse chemical and physical agents can alter cellular functions associated withoxidative metabolism, thus stimulating the production of reactive oxygen species (ROS)and reactive nitrogen intermediates (RNI) in planktonic bacterial physiology. However,more research is necessary to determine the precise role of cellular stress in biofilm. Thepresent study was designed to address the issues 26 of Staphylococcus aureus biofilmformation with respect to the generation of oxidative and nitrosative stress.We studied three pathogenic S. aureus clinical strains and an ATCC one exposedto a different range of culture conditions (time, temperature, pH, reduction andatmospheric conditions) by quantitative methods of biofilm detection.We observed that cellular stress could be produced inside biofilms, therebyaffecting their growth, resulting in an increase of ROS and RNI production, and a decreaseof the extracellular matrix under unfavorable conditions. These radical oxidizers couldthen accumulate in an extracellular medium and thus affect the matrix.These results contribute to a better understanding of the processes that enableadherent biofilms to grow on inert surfaces and lead to an improved knowledge of ROSand RNI regulation, which may help to clarify the relevance of biofilm formation inmedical devices.