IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
MOLECULAR CHARACTERIZATION AND IN SITU DETECTION OF BACTERIAL COMMUNITIES
Autor/es:
FERRERO, M. A.; MENOYO, E.; LUGO, M.; NEGRITTO, M. A.; FARÍAS, M. E.; ANTON, A.M.; SIÑERIZ, F.
Revista:
JOURNAL OF ARID ENVIRONMENTS
Editorial:
ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD
Referencias:
Año: 2010 p. 1 - 9
ISSN:
0140-1963
Resumen:
Puna is a harsh bio-geographical region in South America that characterized by distinct plant
diversity, high aridity and irradiance. In the present study, rhizospheric bacterial communities were
analyzed along an altitudinal gradient in this region while considering the different photosynthesis
pathways (C3/C4) of the native grasses. This analysis included cultivation-dependent and -
independent approaches such as PCR-DGGE analysis of the 16S rRNA genes and FISH. DGGE revealed
that the band richness differed along the altitudinal gradient, but that it did not differ significantly
among plants with different metabolic pathways. Overall, we found that Bacillus and Pseudomonas
were the dominant phylotypes based on the DGGE analysis. Additionally, nearly 80% of the species
identified by PCR were also identified using the cultivation method. FISH analysis revealed that the
Gammaproteobacteria and Actinobacteria were the dominant bacterial groups, followed by Archaea.
Finally, low bacterial diversity was detected in samples collected from all heights, possibly due to the
harsh environment. Overall, evaluation of the results obtained using the two different approaches
revealed that the culture method is efficient for screening of the bacterial community in Puna
rhizospheric soils. Additionally, we discuss possible effects of Puna rhizospheric bacteria on the
protection of native grasses and nutrient capture.