IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Genomic diversity in Solanum sect. Acanthophora (Solanaceae).
Autor/es:
CHIARINI F.; URDAMPILLETA J. D.; LAS PEÑAS M. L.
Revista:
PLANT SYSTEMATICS AND EVOLUTION
Editorial:
SPRINGER WIEN
Referencias:
Lugar: Viena; Año: 2014 vol. 300 p. 113 - 125
ISSN:
0378-2697
Resumen:
In order to study the evolution of different cytogenetic characters in species of Solanum sect. Acanthophora, in relationship to the known phylogeny for this group, the following techniques were used: CMA/DAPI chromosome banding; fluorescent in situ hybridization with probes for the 18-5.8-26S and the 5S rDNA genes in mitotic chromosomes; nuclear DNA quatification by flow cytometry. Depending on the species, 2-6 of the 12 basic chromosome pairs were identified. All species presented one chromosome pair bearing a 18-5.8-26S signal, in synteny with one chromosome pair (exceptionally two) with a 5S signal, and species-specific heterochromatic bands pattern. The techniques employed allowed us to stablish two species groups within Acanthophora: one with small, symmetric chromosomes, few heterochromatin and the lesser DNA content, and another, at the bottom of the phylogenetic tree, with larger, more asymmetric chromosomes, more heterochromatin CMA+/DAPI- (associated to NOR or not) and a higher DNA content. Increased karyotype asymmetry would be associated to an increase in the amount of heterochromatin and in the DNA content. The trend within Acanthophora would be to a loss of heterochromatin, a reduction of chromosome size and an increase in symmetry.