IMBIV   05474
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Short-term effects of combined iprodione and vermicompost applications on soil microbial structure
Autor/es:
VERDENELLI R.A.; LAMARQUE A.L.; MERILES J.M.
Revista:
SCIENCE OF THE TOTAL ENVIRONMENT
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2012 vol. 414 p. 210 - 219
ISSN:
0048-9697
Resumen:
The use of compost amendments to bioremediate potential organic pollutants in agricultural soils has recently
become an increasingly important field of research. Although several fungicides have been extensively
used to control a wide range of soil-borne fungal diseases, little is known about the impact of applying
these pesticides on the structure and function of microbial communities in soils amended with vermicompost.
The aim of this study was to evaluate the effect of a combined treatment of iprodione and vermicompost
on soil microbiological parameters under laboratory conditions. The study was carried out on agricultural and
grassland soils to identify the effect of iprodione application at field rate (FR) and 10-times FR (10FR) with
and without vermicompost (VCH) on iprodione breakdown, fluorescein diacetate activity (FDA), total fatty
acid methyl ester (FAME) profiles, total protein content, and protein profiles by using sodium dodecyl
sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Our results indicate that the addition of vermicompost
decreased the iprodione breakdown at days 30 and 60 in non-sterilised agricultural soil and at 60 days in
sterilised and non-sterilised grassland soil. Independent of vermicompost amended treatments, iprodione
was found to mainly alter microbial communities after 30 days of incubation. On day 30, separation between
communities treated with iprodione 10FR and iprodione 10FR+VCH treatments were well defined in both
agricultural and grassland soils. Within each soil type, our results showed no difference in the total protein
content. However, the protein content in the grassland soil was clearly higher than in the agricultural soil.
SDS-PAGE gels revealed that the treatments applied to the agricultural soil using iprodione at the highest
dosages (iprodione 10FR and iprodione 10FR+VCH) resulted in an alteration of the band pattern. In conclusion,
the experiments revealed that the addition of vermicompost may decrease the breakdown of iprodione
in soils. Furthermore, elevated dosages of iprodione may potentially affect the microbial community structure
and diversity of the soil, which may lead to the deterioration of soil quality and fertility.field of research. Although several fungicides have been extensively
used to control a wide range of soil-borne fungal diseases, little is known about the impact of applying
these pesticides on the structure and function of microbial communities in soils amended with vermicompost.
The aim of this study was to evaluate the effect of a combined treatment of iprodione and vermicompost
on soil microbiological parameters under laboratory conditions. The study was carried out on agricultural and
grassland soils to identify the effect of iprodione application at field rate (FR) and 10-times FR (10FR) with
and without vermicompost (VCH) on iprodione breakdown, fluorescein diacetate activity (FDA), total fatty
acid methyl ester (FAME) profiles, total protein content, and protein profiles by using sodium dodecyl
sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Our results indicate that the addition of vermicompost
decreased the iprodione breakdown at days 30 and 60 in non-sterilised agricultural soil and at 60 days in
sterilised and non-sterilised grassland soil. Independent of vermicompost amended treatments, iprodione
was found to mainly alter microbial communities after 30 days of incubation. On day 30, separation between
communities treated with iprodione 10FR and iprodione 10FR+VCH treatments were well defined in both
agricultural and grassland soils. Within each soil type, our results showed no difference in the total protein
content. However, the protein content in the grassland soil was clearly higher than in the agricultural soil.
SDS-PAGE gels revealed that the treatments applied to the agricultural soil using iprodione at the highest
dosages (iprodione 10FR and iprodione 10FR+VCH) resulted in an alteration of the band pattern. In conclusion,
the experiments revealed that the addition of vermicompost may decrease the breakdown of iprodione
in soils. Furthermore, elevated dosages of iprodione may potentially affect the microbial community structure
and diversity of the soil, which may lead to the deterioration of soil quality and fertility.field rate (FR) and 10-times FR (10FR) with
and without vermicompost (VCH) on iprodione breakdown, fluorescein diacetate activity (FDA), total fatty
acid methyl ester (FAME) profiles, total protein content, and protein profiles by using sodium dodecyl
sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Our results indicate that the addition of vermicompost
decreased the iprodione breakdown at days 30 and 60 in non-sterilised agricultural soil and at 60 days in
sterilised and non-sterilised grassland soil. Independent of vermicompost amended treatments, iprodione
was found to mainly alter microbial communities after 30 days of incubation. On day 30, separation between
communities treated with iprodione 10FR and iprodione 10FR+VCH treatments were well defined in both
agricultural and grassland soils. Within each soil type, our results showed no difference in the total protein
content. However, the protein content in the grassland soil was clearly higher than in the agricultural soil.
SDS-PAGE gels revealed that the treatments applied to the agricultural soil using iprodione at the highest
dosages (iprodione 10FR and iprodione 10FR+VCH) resulted in an alteration of the band pattern. In conclusion,
the experiments revealed that the addition of vermicompost may decrease the breakdown of iprodione
in soils. Furthermore, elevated dosages of iprodione may potentially affect the microbial community structure
and diversity of the soil, which may lead to the deterioration of soil quality and fertility.fluorescein diacetate activity (FDA), total fatty
acid methyl ester (FAME) profiles, total protein content, and protein profiles by using sodium dodecyl
sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Our results indicate that the addition of vermicompost
decreased the iprodione breakdown at days 30 and 60 in non-sterilised agricultural soil and at 60 days in
sterilised and non-sterilised grassland soil. Independent of vermicompost amended treatments, iprodione
was found to mainly alter microbial communities after 30 days of incubation. On day 30, separation between
communities treated with iprodione 10FR and iprodione 10FR+VCH treatments were well defined in both
agricultural and grassland soils. Within each soil type, our results showed no difference in the total protein
content. However, the protein content in the grassland soil was clearly higher than in the agricultural soil.
SDS-PAGE gels revealed that the treatments applied to the agricultural soil using iprodione at the highest
dosages (iprodione 10FR and iprodione 10FR+VCH) resulted in an alteration of the band pattern. In conclusion,
the experiments revealed that the addition of vermicompost may decrease the breakdown of iprodione
in soils. Furthermore, elevated dosages of iprodione may potentially affect the microbial community structure
and diversity of the soil, which may lead to the deterioration of soil quality and fertility.files, total protein content, and protein profiles by using sodium dodecyl
sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Our results indicate that the addition of vermicompost
decreased the iprodione breakdown at days 30 and 60 in non-sterilised agricultural soil and at 60 days in
sterilised and non-sterilised grassland soil. Independent of vermicompost amended treatments, iprodione
was found to mainly alter microbial communities after 30 days of incubation. On day 30, separation between
communities treated with iprodione 10FR and iprodione 10FR+VCH treatments were well defined in both
agricultural and grassland soils. Within each soil type, our results showed no difference in the total protein
content. However, the protein content in the grassland soil was clearly higher than in the agricultural soil.
SDS-PAGE gels revealed that the treatments applied to the agricultural soil using iprodione at the highest
dosages (iprodione 10FR and iprodione 10FR+VCH) resulted in an alteration of the band pattern. In conclusion,
the experiments revealed that the addition of vermicompost may decrease the breakdown of iprodione
in soils. Furthermore, elevated dosages of iprodione may potentially affect the microbial community structure
and diversity of the soil, which may lead to the deterioration of soil quality and fertility.fined in both
agricultural and grassland soils. Within each soil type, our results showed no difference in the total protein
content. However, the protein content in the grassland soil was clearly higher than in the agricultural soil.
SDS-PAGE gels revealed that the treatments applied to the agricultural soil using iprodione at the highest
dosages (iprodione 10FR and iprodione 10FR+VCH) resulted in an alteration of the band pattern. In conclusion,
the experiments revealed that the addition of vermicompost may decrease the breakdown of iprodione
in soils. Furthermore, elevated dosages of iprodione may potentially affect the microbial community structure
and diversity of the soil, which may lead to the deterioration of soil quality and fertility.