INVESTIGADORES
PEICHOTO Maria elisa
congresos y reuniones científicas
Título:
Patagonfibrase modifies protein expression of tissue factor and protein disulfide isomerase in rat skin
Autor/es:
MARÍA ELISA PEICHOTO; MARCELO LARAMI SANTORO
Lugar:
Montpellier
Reunión:
Congreso; 62ND ANNUAL MEETING OF THE SCIENTIFIC AND STANDARDIZATION COMMITTEE OF THE INTERNATIONAL SOCIETY ON THROMBOSIS AND HAEMOSTASIS; 2016
Institución organizadora:
International Society on Thrombosis and Haemostasis
Resumen:
Background: Patagonfibrase is a hemorrhagic metalloproteinase isolated from the venom of the South American rear-fanged snake Philodryas patagoniensis, and is an important contributor to local lesions inflicted by this species. The tissue factor (TF)-factor VIIa complex, besides triggering the coagulation cascade, has been demonstrated to be involved in inflammatory events.Aims: This study was performed to determine whether patagonfibrase affects the local expression of two proteins having important roles in hemostasis and inflammation: tissue factor (TF) and protein disulfide isomerase (PDI).Methods: Patagonfibrase (60 lg/kg) was administered s.c. to rats, and after 3 h blood was collected to evaluate hemostasis parameters, and skin fragments close to the site of injection were taken to assess TF and PDI expression.Results: Patagonfibrase did not alter blood cell counts, plasma fibrinogen levels, or levels of TF activity in plasma. However, by semiquantitative Western blotting, patagonfibrase increased TF expression by 2-fold, and decreased PDI expression by 3-fold in skin samples. In agreement, by immunohistochemical analyses, prominent TF expression was observed in the subcutaneous tissue.Conclusions: Patagonfibrase affects the local expression of TF and PDI without inducing any systemic hemostatic disturbance, although evidencing that they may be involved in the inflammatory events induced by hemorrhagic metalloproteinases. Once antivenom therapy is not totally effective to treat the local injury induced by snake venoms, modulation of the activity and expression of TF and/or PDI might become a strategy for treating snake envenomation.