INVESTIGADORES
PEICHOTO Maria elisa
congresos y reuniones científicas
Título:
Responsability of IgG anti-PLA2 in the reaction between P. patagoniensis venom & anti-crotalic serum
Autor/es:
JUAN PABLO RODRÍGUEZ; MARÍA ELISA PEICHOTO; LAURA LEIVA; OFELIA ACOSTA
Lugar:
Rosario (Argentina)
Reunión:
Otro; XLII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2006
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Resumen:
Cross reactivity among viperid venoms and their commercial sera is perfectly known, whereas there is little information about immunoreactivity between colubrid venoms and anti-viperid sera. Herein, the aim of the present work was to detect if the anti-crotalic serum is able to react with the venom of the opisthoglyphous colubrid snake Philodryas patagoniensis. In order to analize this, immunodiffusion assay was carried out, which showed a single precipitation line when P. patagoniensis venom was tested with commercial anti-crotalic serum. In order to investigate if IgG anti-PLA2 was responsible for this cross reaction, immunodiffusion and western blotting assays were carried out with this antibody. Both tests demonstrated that IgG anti-PLA2 does not participate in this cross reactivity. Moreover, P. patagoniensis venom showed to be devoid of PLA2 activity. These preliminary studies confirm that proteins from P. patagoniensis venom do not have common antigenic sites with the main component of crotalic venom, the PLA2 from the crotoxin complex. The identity of those antigens shared by both venoms will be further investigated.