INVESTIGADORES
PEICHOTO Maria elisa
congresos y reuniones científicas
Título:
Patagonstatin, a potent inhibitor of collagen-induced platelet aggregation isolated from Philodryas patagoniensis colubrid snake venom
Autor/es:
MARÍA ELISA PEICHOTO; FLÁVIO TAVARES; LAURA LEIVA; OFELIA ACOSTA; ANITA MITIKO TANAKA-AZEVEDO; MARCELO LARAMI SANTORO
Lugar:
Riberão Preto (Brasil)
Reunión:
Congreso; 38º Congreso Brasileño de Farmacología y Terapéutica Experimental; 2006
Institución organizadora:
Sociedad Brasileña de Farmacología y Terapéutica Experimental
Resumen:
Introduction: Venoms of Colubridae snakes are a rich source of novel compounds, which may have applications in medicine and biochemistry. Methods and Results: Patagonstatin is a single-chain protein isolated from Philodryas patagoniensis venom, with a molecular mass of 25.3 kDa under non-reducing conditions and 27.4 under reducing conditions. Purification of this protein was accomplished by chromatography on Mono-Q and Phenyl-Sepharose columns. Patagonstatin was homogenous by SDS-PAGE and hydrolyzed neither azocasein nor fibrinogen. Mild hemorrhage developed in mouse skin after i.d. injection of patagonstatin (20 µg of protein produced a hemorrhagic spot of 2.40 mm in diameter). Twenty micrograms of this protein induced edema, reaching its maximum after 2 h of s.c. injection. Patagonstatin showed no platelet pro-aggregating activity per se, but it inhibited collagen-induced platelet aggregation, with an IC50 of 9.1 nM. Ristocetin- and ADP-induced platelet aggregations were not inhibited by this protein. Discussion: This is the first report on the isolation and characterization of a platelet aggregation inhibitor from the venom of a colubrid snake. Due to its potent action on collagen-induced platelet aggregation, patagonstatin may be used on studies of platelet physiology.