INVESTIGADORES
PEICHOTO Maria elisa
congresos y reuniones científicas
Título:
Comparison of the chromatographic profiles of Philodryas baroni and Philodryas patagoniensis venoms
Autor/es:
LAURA LEIVA; MARÍA ELISA PEICHOTO; CAROLINA GAY; SILVANA MARUÑAK; OFELIA ACOSTA; LAURA REY
Lugar:
Tandil (Buenos Aires)
Reunión:
Otro; XXXIV Reunión Anual de la Asociación Argentina de Farmacología Experimental; 2002
Institución organizadora:
Sociedad Argentina de Farmacología Experimental
Resumen:
The salival secretion of colubrid snakes belonging to the genus Philodryas, considered as not poisonous, has caused serious injuries. Fibrin(ogen)olytic enzymes, which are responsible of  tissue injuries and hemostatic alterations in bitten individuals, have been isolated from P. olfersii venom. This work presents a preliminary study about P. baroni and patagoniensis venoms, which inhabit Argentina and whose secretions have not been studied yet. The profiles of elution are compared by ion exchange chromatography (DEAE-celulose equilibrated and eluted with 10 mM buffer Tris-HCl, pH=7,5).  The eluted proteins were followed by the measurement of A280, and the proteolytic activity was measured on casein. Crude venoms of both epecies showed a strong proteolytic activity (P. patagoniensis=253 U/mg; P. baroni=88 U/mg). The elution profile of P. patagoniensis venom showed a peak corresponding to unbound proteins (basic proteins) with proteolytic activity; and three peaks corresponding to fractions that eluted after raising the ionic strength of the elution buffer, of wich only the most acidic peak did not show proteolytic activity. The elution profile of P. baroni venom showed a peak corresponding to unbound proteins (basic proteins) with proteolytic activity; and two peaks corresponding to fractions that eluted after raising the ionic strength of the elution buffer, wich did not show proteolytic activity. From this comparative study, we can make evident that both venoms differ in their compositions; detecting proteolytic enzymes, which award toxicity to these venoms.